小小骷髅
Coated ELISA plates with COX-2 anti-rat monoclonal antibody,then add biotinylated anti-rat COX-2 after the standard and samples of COX-2 combined with the monoclonal antibody.And the Streptavidin which marked by Horse radish peroxide enzyme combined with biotin. Then joins substrate driving fluid obviously blue color.Finally, add the termination of liquid sulfuric acid,measure the value of OD at 450nm,then draw the standard curve in a coordinate system which useingstandard 20,10,5,2.5,1.25,0.625,0.312, 0 ng / ml for the abscissa, OD value for longitudinal coordinates.And then, identify the corresponding COX-2 content.
白白桃花
教你一个简单的方法参考同类文献的摘要翻译方法。还有可以参考外文的写法。另外可以使用google的翻译功能，不过需要重新整理。google的翻译转录如下（你自己整理吧）Objective: To investigate the serum protein electrophoresis in patients with liver cirrhosis spectrum Change for clinical diagnosis and treatment of the provision of reference materials. Methods: The United States Helena SPIFE 2000 agarose electrophoresis Automatic Determination of 138 cases and 50 cases of liver cirrhosis in patients with normal serum protein ratio. Results: The patients with liver cirrhosis of serum protein electrophoresis of each component compared with the normal result, there is significant difference. Conclusion: in patients with cirrhosis of the serum protein electrophoresis analysis must have the characteristics of cirrhosis are the diagnosis, prognosis and efficacy of a good indicator.
晓晓小同学
With COX-2 anti-rat monoclonal antibody coated on the ELISA plate, and standard samples with the COX-2 monoclonal antibody, adding biotinylated anti-rat COX-2, the formation of immune complexes in connection board , Horseradish peroxidase labeled with Streptavidin biotin, adding at the end of the work-blue LCD, plus the final termination of the liquid sulfuric acid, in the Department 450nm measuring OD value, COX-2 concentration and OD value in direct proportion to the standard Commodities 20,10,5,2.5,1.25,0.625,0.312, 0 ng / ml for the abscissa, OD value of longitudinal coordinates, draw the standard curve, corresponding to identify COX-2 content
好吃不懒做也
上面三位都不够简练，其实多数人何尝不是如此呢？医学作为一门Subject,“Medicine”一次足矣！且最常用。人们都知道这是“医药”，却忽视了它的另一含义。一道题是一道题，你有什么问题再问上来，你的补充问题其实网络上比比皆是，且太不具体的东西我无从回答。
嘟嘟200907
With COX-2 anti-rat monoclonal antibody coated on the ELISA plate, and standard samples with the COX-2 monoclonal antibody, adding biotinylated anti-rat COX-2, the formation of immune complexes in connection board , Horseradish peroxidase labeled with Streptavidin biotin, adding at the end of the work-blue LCD, plus the final termination of the liquid sulfuric acid, in the Department 450nm measuring OD value, COX-2 concentration and OD value in direct proportion to the standard Commodities 20,10,5,2.5,1.25,0.625,0.312, 0 ng / ml for the abscissa, OD value of longitudinal coordinates, draw the standard curve, corresponding to identify COX-2 content. (Translated into English)
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