缠藤小妖
The quantity of heat-resistant lipases produced by psychrotrophic is one of the main factors that influence the quality of law milk,a mass of heat-resistant would shorten the shelf life of dairy products,and it's possible to arise the condition of bitter package and composition changed.This article did research on the purification of heat-resistant lipases in psychrotrophic,adopted the means of ammonium sulfate precipitation、Ion exchange resin chromatography、gel filtration chromatography to purify the ECPase of Pseudomonsa fluorescens and analyse the features.The results show:the most appropriate temperature of this lipases is 30℃，the most appropriate PH is 7.0,Fe have some effects of stimulation to Protease activity,PMSF、EDTA have no distinct suppression to Protease activity,but the DDT cause a decrease of Protease activity.This lipases have a strong capacity of heat-resistant.
吃那么一天
好累啊，不过有些太专业的我也不能肯定翻译得特别恰当，尤其是那个苦包...Eosinophilic cold bacteria（嗜冷菌） is one of the main factors that affects the quality of raw milk , because of its existence and produce a lot of heat-resistant protease（耐热蛋白酶）will cause shortening of shelf life of dairy products, and may appear bitter bag（苦包（我不太懂什么是苦包））, the state of organization change. This essay studied the purification in eosinophilic cold bacteria protease in raw milk, and by ammonium sulfate precipitation, ion exchange resin chromatography, gel filtering chromatography method on fluorescence pseudomonas exocellular protease and characteristic analysis on the purification. The results show that the optimal temperature for the enzyme is 30 ℃, the optimal pH is7.0, Fe 2+ has certain stimulating effect for the activity of protease, without apparent inhibition to PMSF, EDTA protease actiity and DTT make enzymes living dropped. This enzyme has strong ability of resisting heat.
