The purpose:According to the physical and chemical properties of shikonin on the total pigment shikonin in Lithospermum to optimize the extraction The method:First of all, with 95%, respectively, ethanol, petroleum ether (60 ℃ -90 ℃), acetone, chloroform and other organic solvents for extraction of total pigment shikonin ability to inspect, the better the selection of solvent extraction, and then choose extraction dynamic orthogonal extraction were optimized in order to Shikonin total extraction rate for the pigment inspection targets, optimization of the extraction of the best Results: the best optimization by orthogonal experiments and extraction of solvent extraction methods, colorimetric assay, statistical analysis to determine the total pigment shikonin the optimum Conclusion: petroleum ether (60 ℃ -90 ℃) as extraction solvent, extraction dynamic, temperature controlled at 50 ℃, plus 10 times the dosage of Health of the solvent, extracting twice an hour extraction, this is Shikonin the best total pigment Key words: Boraginaceae; Shikonin total pigment; extraction process; orthogonal experiment; Colorimetry; dynamic extraction method
Different origin of betelnut alkali betel nutUsing spectrophotometry in different habitats (hainan, guangdong, yunnan WZS xuwen in fujian, estuary with varieties of shaoxing-jiaxing Ann) of alkali content of betelnut betel Experiments show that different alkali content of betelnut betel nut there is some difference with linear regression equation, A 160 = 10-3C - 0617 measured in four different alkali content of betelnut betel nut is as follows: the 426% wuzhishan hainan, guangdong, yunnan, estuary 377% xuwen 314%, fujian szabics 344% AKey words: betelnut; Betelnut alkali content, Different origin, Spectrophotometric method