Goal: Understood the thyroxine acceptor β△ gene in big mouse different organization distributed characteristic， is familiar with with PT-PCR analyzes the Method: TRβ△ the gene has the expression in many kinds of organizations， in which liver， the brain， the skeleton muscle， the heart， the kidney expression are Withdraws big mouse many place organizations mRNA， withdraws mRNA is the template counter duplication synthesizes the cDNA first And expands take it as template PCR increases the goal gene which TRβ△ the double strand cDNA basis copies to design two to direct the Uses fluorescence quota PCR the method to carry on the quantitative Finally: The extraction organizes total RNA， OD260/OD280 to be situated between between 7-0， OD260/230 is situated between between 5- The agarose gel electrophoresis appraises PCR to expand increases the result to demonstrate that， has the goal about anticipated 160bp to expand approximately increases the product belt TRβΔ PCR product the agarose gel electrophoresis appraisal result to indicate， expands the DNA molecular weight size after PCR which increases is the 176bp goal The goal gene and the steward gene quantitative determination， it expands increases the curve and the curve of solubility has not seen the mixed peak， simultaneously melts the tune prominent peak correspondence Tm value with to expand increases the product theory Tm to be Conclusion: The findings showed TRβ△ the gene has certain spatial distribution characteristic in the mRNA level expression: The liver， the brain， the spleen， in the kidney organization has this gene expression， also in the spleen expression quantity is higher than other organizations
1 What is eukaryotic cella Eukaryotic cell refers to cell that has eukaryotic nucleus(nucleus that is surrounded by nuclear membrane)Its chromosome number is more than one and it is able to carry out It can also carry out protoplasm movement and Photosynthesis and oxidation and phosphorylation are carried out by chloroplasts and mitochondria Except for the cells of bacteria and blue-green algae，all the animal cells and plant cells are eukaryotic Organisms made up of eukaryotic cells are called eukaryotic In the nucleus of eukaryotic organisms，proteins like DNA and histone，etc together make up the structure of The nucleolus can be observed in the The memebrane system inside the cytoplasm are very advanced，including celluar organelles like the endoplamic network，Golgi apparatus，mitochondria and cytolysosome，They implement specific functions b endoplasmic reticulum:a continuous pipe system surrounded by The surface of rough endoplasmic reticulum(RER) contains ribosomes which take part in the synthesis and processing of proteins;there is no ribosome on the surface of smooth endoplasmic reticulum (SER) to take part in the synthesis of Membrane-bound organelles or membrane-bound (这句话不完整)C Memerane-bound organelles refer to all the organelles inside the cytoplasm that have membrane structures，including nucleus，endoplasmic reticulum，Golgi apparatus，cytolysosome，vesicles，mitochondria，chloroplasts and peroxide catalase，Since they are all closed membrane structures，they have some space d Lysosome is an organelle inside the cytoplasm that is surrounded by single fatty protein membranes and contains a series of acidic It is an organelle inside the cell that has single membrane Lysosome contains many types of hydrolases which can break down many Lysosome is likened to the enzyme warehouse or digestive system inside the Lysosome has two functions:one is that it can fuse with food vesicles，digest large particles like food engulfed by the cell or pathogenic bacteria，etc into large molecules，and eject residues out of the cell;the other function is that druing the celluar differentiation process，some old organells and molecules，etc are engulfed into and digested by This is due to the need of the organism to re-organise 2 describe red blood cellsWhen the red blood cells mature，it no longer has any membrane structures like nuclear memebrane and mitochondrion 3 Eukaryotic red blood cellsa Reb blodd cells are rounded，nucleusless cells within the human bone marrow which are biconcave and thick at the They have an average radius of 5 mirometers，a thickness of 7 micrometers，an average capacity of 83 cube micrometers，and an average surface area of approximately 145 square About 44% of the blood 's capacity are cellular components，most of which are red blood In one microliter of blood，males have an average of 5000 thousands，females have an average of about 4600 Other than water，haemoglobins are the main components of red blood
Stria albicans the disease that he|she's mosquito spreads and their damage the main body of a book has been introduced He|she's mosquito complies with the lines having introduced the home and abroad dialogue moreover resistance to drugs， a few that the information compound carries out on whose aspect such as affecting ， rotating a gene to study study current The main body of a book has been set forth at the same time having a dialogue lines some control method of he|she's Avoid meeting an agent if insecticide， runs quickly ， lure an agent to wait for chemistry control method ， the algae ， eat mosquito fish wait for living things wait for aspect under the control of and their comprehensive ecological And a few defects there existing in middle in these control The purpose introducing that the thesis studies finally and their significance: Find out the most effective mosquito luring an agent ， laying down fine basis combining with inducing mosquito light to wait for a variety of means to reach the exploitation trapping and killing effect best ， luring an agent for the carrying out and high-effect mosquito owing aspect the job studying further in the days to
Abstract: the market demand for the buckeye， but because the buckeye conventional breeding way is seed propagation， reproductive slower， plus the buckeye rate is low and seeds， seedlings to inactivation rate is weak， so the unearthed， reproduction rate low， difficult to meet the market Therefore， from the flowering length， the effect of the climatic conditions and take the morphological structure， flowering length to the pollen of energy， energy， the seed vigor of pollen influence on some physiological indexes of determination to proven buckeye rate， low birth rate， low reason for improving the buckeye setting out the theoretical basis and reproduction rate， in order to improve the buckeye breeding take measures to meet the demand of the The growth of the buckeye flowering characteristicsKeywords: buckeye， Growth flowering， Dynamic measurement
