专业术语我可能翻译得不太好，仅供参考！中文摘要核盘菌引起的作物菌核病是世界性分布的一种病害。本论文以分离自陕西省核盘菌SX466菌株为研究材料，通过分析该菌株的生物学特性，发现相比于正常菌株Ep-1PNA367，菌株SX466在PDA及胡萝卜培养基上不能产生菌核，生长速度减弱（？？cm），致病力衰退，是一株具有典型弱毒现象（hypovirulence）的弱毒菌株。通提取弱毒菌株SX466的dsRNA及电泳分析，表明该菌株中含有？？条dsRNA片段，弱毒现象可能与dsRNA存在具有相关性。本论文的完成为研究核盘菌的致病机理及真菌中dsRNA介导的弱毒特性提供了很好的研究材料及基础。关键字：核盘菌；弱毒菌株；生物学特性；dsRNAAbstract:Sclerotiorum disease， which is a type of crop desease caused by Sclerotinia Sclerotiorum distributes all over the With the SX466 of Sclerotinia Sclerotiorum isolated from Shan xi province as researching objective in this paper， it is observed through analyses of the biological characteristics of isolate of SX466 that compared with the normal isolate Ep-1PNA367， isolate SX466 does not produce sclerotium on PDA and carrot nutrient medium， its growth rate(??cm) is reduced and vieulence degenerated， which makes isolate SX466 a hypovirulent strain with a typical phenomenon of Via dsRNA extration of isolate SX466 and electrophoretic analysis， it is indicated that ?? dsRNA fragments is contained in this isolate and hypovirulence may have correlation with the existence of dsRNA Valuable researching material and basis are provided for the research of pathogenic mechanism of Sclerotinia Sclerotiorum as well as the dsRNA-mediated hypovirulence in fungus by the completion of this Keywords: Sclerotinia Sclerotiorum hypovirulent strain biological characteristics dsRNA
